Loganin

Rat interleukin (IL)-1β, IL-6, tumor necrosis factor alpha (TNF-α) enzyme-linked immunosorbent assay (ELISA) kit was obtained from Multi Science Biotechnology Co., Ltd. (Hangzhou, China). Reference standards of ferulic acid, atractylodin, tanshinone, astragaloside, hyperoside, loganin, gallic acid, morroniside, allantoin, oleanic acid, ginsenoside Rb1, ginsenoside Re, ginsenoside Rg1, paeoniflorin, vaccarin, pyrrolidinium, leonurine, and catalpol were obtained from Sichuan Weikeqi Biological Technology Co., Ltd. (Sichuan, China).

extracted and purified by our laboratory from the aerial portion of Sibiraea angustata and structural identification was done by the spectroscopic analysis. Andrographolide (≥98%), Loganin (≥98%) and Gastrodin (≥ 98%) were purchased from Sichuan Weikeqi Biological Technology Co., Ltd. (Chengdu, China). Deionized ultrawater was used from a laboratory purification system.

4-Caffeoylquinic acid(4-CQA), chlorogenic acid(3-CQA), 3,5-dicaffeoylquinic acid(3,5-diCQA), loganic acid, loganin, sweroside, dipsacoside B and asperosaponin VI were purchased from Sichuan Weikeqi Biotechnology Co. Chloramphenicol and glycyrrhetinic acid were purchased from Shanghai Yuanye Biological Technology Co.

authenticated by Prof.Cai Baochang, Director of Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine. Standard substances were purchased from Sichuan Weikeqi Biotechnology Co located in Chengdu City, Sichuan Province, including 4-caffeoylquinic acid (4-CQA), chlorogenic acid (3-CQA), 3,5-dicaffeoylquinic acid (3,5-diCQA), loganic acid, loganin, and asperosaponin VI.

Standard compounds, including 4-caffeoylquinic acid (4-CQA), chlorogenic acid (3-CQA), 3,5-dicaffeoylquinic acid (3,5-diCQA), loganic acid, loganin, sweroside, dipsacoside B, and asperosaponin VI were purchased from Sichuan Weikeqi Biotechnology. Chloramphenicol and glycyrrhetinic acid were obtained from Shanghai Yuanye Biological Technology. The purity of each standard substance was more than 98%. HPLC-grade acetonitrile was obtained from Merck.

Standard substances, including 4-caffeoylquinic acid (4-CQA), loganic acid, 3,4-dicaffeoylquinic acid (3,4-diCQA), 3,5-dicaffeoylquinic acid (3,5-diCQA), loganin, sweroside and asperosaponin VI were purchased from Sichuan Weikeqi Biotechnology Co. Dipsacussaponin B, asperosaponin V and dipsacoside A were obtained from Nanjing Shizhou Biological Technology Co. The purity of each standard substance was >98%. Their chemical structures are shown in Supplementary Figure S1.