异绿原酸C/4，5-二咖啡酰奎宁酸/4,5-二-O-咖啡酰奎宁酸

All other chemicals and extraction solvents used were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Ten chemical standards were purchased from Weikeqi Biological Technology Co, Ltd. (Sichuan, China), including chlorogenic acid (3-CQA), cryptochlorogenic acid (4-CQA), neochlorogenic acid (5-CQA), isochlorogenic acid A (3,5-diCQA), isochlorogenic acid B (3,4-diCQA), isochlorogenic acid C (4, 5-diCQA), eriodictyol-7-O-β-d-glucoside, luteolin-7-O-β-d-glucoside, 5, 7, 4′-trihydroxy-6, 3′, 5′-trimethoxyflavone, eupatilin (purity ≥ 99%). In addition, eriodictyol-7-O-β-d-glucuronide was isolated and purified from Jumi in the laboratory (>98%). All of the stock and working standard solutions were stored at 4 °C until used for the experiments.

The reference standards of isorhmnetin (3), diosmetin (6), isochlorogenic acid A (7), kaempferol (10), quercetin (14), isochlorogenic acid C (15), apigenin (19), forsythin (20), chlorogenic acid (23), liquiritin (25), luteolin-7-O-glucoside (26), astragalin (28), luteolin (29), buddleoside (31), quercetin-3-O-glucoside (33), amygdalin (37), caffeic acid (38), forsythoside A (39), glycyrrhizic acid (40), ferulic acid (42), rutin (44), hyperoside (50), and platycodin D (51) were purchased from Sichuan Weikeqi Biological Technology, Co., Ltd. (Chengdu, China). Mulberry leaf, chrysanthemum, Fructus Forsythiae, licorice, Semen Armeniacae Amarum, Platycodi Radix and Phragmitis Rhizoma

Tseng and their structures were fully characterized by chemical and spectroscopic methods. The other standards, namely protocatechuic acid (3,4-DA, R3), rutin (R4), neochlorogenic acid (5-CQA, C1), chlorogenic acid (3-CQA, C2), cryptochlorogenic acid (4-CQA, C3), caffeic acid (CA, C4), isochlorogenic acid B (3,4-CQA, C5), isochlorogenic acid A (3,5-CQA, C6) and isochlorogenic acid C (4,5-CQA, C7) were purchased from the National Institute for Food and Drug Control (Beijing, China) or Sichuan Weikeqi Biological Technology Co., Ltd. (Qingjiang, Zhonglu, China) Purity analysis proved to be above 98% by HPLC, and their chemical structures are listed in Figure 7.

purchased from National Institute for Food and Drug Control (Beijing, China). Neochlorogenic acid (5-CQA, C1), cryptochlorogenic acid (4-CQA, C3), isochlorogenic acid B (3,4-diCQA, C5), isochlorogenic acid A (3,5-diCQA, C6) and isochlorogenic acid C (4,5-diCQA, C7) were bought from Weikeqi Biological Technology Co., Ltd (Sichuan, China). The reference substances of 6-hydroxy-7.7α-dihydrobenzofuran-2(6H)-one (R1), hydroxyl-casein (R2), latifoloside G (K1), kudinoside G (K2), kudinoside A (K3), kudinoside E (R5)

were supplied from Chengdu Pufeide Biological Technology Co., Ltd. (Sichuan, China). The standards of scutellarin, isochlorogenic acid A, isochlorogenic acid C, and apigenin were purchased from Sichuan Weikeqi Biological Technology Co., Ltd. (Sichuan, China). The internal standard gallic acid (IS1) was purchased from the National Institute for Control of Pharmaceutical

The reference standards of chlorogenic acid, forsythiaside A, isochlorogenic acid C, liquiritin, phillyrin, rutin, isoquercitrin, luteoloside and glycyrrhizinate were purchased from Sichuan Weikeqi Biological Technology Co., Ltd (Chengdu, China).

Reference substances of NA (batch no. AB7050442), CGA (batch no. AB7061002), and ICAC (batch no. wkq17120111), all purity ≥98%, were purchased from Sichuan Weikeqi Biological Technology Co., Ltd. (Sichuan, China).

The reference standards of 3,4-DCQA, 3,5-DCQA, 4,5-DCQA were purchased from Sichuan Weikeqi Bio-tech Co., Ltd. (Sichuan, China). Tinidazole (used as internal standard, IS) was purchased from National Institute for the Control of Pharmaceutical and Biological Products

HPLC-grade acetonitrile was obtained from Merck. 5-Caffeoylquinic acid,

3-caffeoylquinic acid, 4-caffeoylquinic acid, 4,5-di-O-caffeoylquinic

acid, 3,4-di-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid

were purchased from Sichuan Weikeqi Biological Technology Co.

Erigeroside, scutellarin, erigoster B, scutellarin A and luteolin 7-Ob-D-glucuronide were obtained from Chengdu Purechem-standard

Co.